HEMATOXYLIN AND EOSIN (H&E)
Used for elucidation of basic histologic features, prior to the use of special stains or immunohistochemical studies as needed; among other features, calcification and microorganisms such as fungi and bacteria may be detected by H&E and confirmed by additional studies
Fontana-Masson (Fig. 30-1)
Stains melanin and argentaffin granules black (nuclei will be red); useful for quantifying melanocytes (e.g., in vitiligo) and in cases of minocycline pigmentary alteration
Also stains Cryptococcus and the phaeohyphomycotic organisms
Grimelius argyrophil stain
Fontana-Masson stains melanin black in this biopsy of minocycline pigmentation (200x) (also see Fig. 30-3).
PAS stain showing dermatophytes in stratum corneum (200x).
Prussian blue stains iron blue (200x). This is a case of minocycline pigmentation, which was also Fontana-Masson positive.
STAINS FOR CONNECTIVE TISSUE COMPONENTS