RT Book, Section A1 Emanuel, Patrick A1 Cavanagh, Martin A2 Emanuel, Patrick A2 Izzard, Mark SR Print(0) ID 1199482893 T1 Slide Preparation and Immunohistochemistry T2 Margin Control Surgery of the Skin: Concepts, Histopathology, and Applications YR 2023 FD 2023 PB McGraw Hill PP New York, NY SN 9781264285990 LK dermatology.mhmedical.com/content.aspx?aid=1199482893 RD 2024/03/28 AB High-quality staining is central to the successful interpretation of pathology slides. In fact, it is probably not advisable to consider doing margin control surgery (MCS) without a well-equipped laboratory and trusted staff. Most laboratories use the hemotoxylin and eosin (HE) stain to colorize slides. This picks up the various tissue and cellular components in different ways, resulting in a highly reproducible artifact allowing assessment of the cellular and tissue morphology. Pathologists use these staining characteristics to make determinations about the features of disease they see microscopically. In a sense, it is medicine’s version of physiognomy, the study of how someone’s personality can be determined just by looking at their facial characteristics. Though it may seem superficial and far less sophisticated than an involved genetic analysis, the centuries-old method of HE staining continues to be the gold standard in diagnostic pathology.